Shimadzu Spectrophotometer UV-1900i

The UV-1900i is a double-beam UV-Vis spectrophotometer using Shimadzu's original LO-RAY-LIGH™ diffraction grating technology. In addition to its high optical performance, the UV-1900i features high resolution, low stray light, high reproducibility, and an ultra-fast scan function. It also has an easy-to-use interface on a color touch-screen display. The UV-1900i is designed to meet the needs of both high performance and usability.

Full Support for Pharmacopeia, GLP/GMP, FDA 21 CFR Part 11 and Other Regulations

Instrument Validation Functions Compliant with JP, USP, and EP

This instrument can not only run checks for nine JIS items, but also those stipulated in the Japanese Pharmacopoeia (JP), United
States Pharmacopeia (USP), and the European Pharmacopoeia (EP). Naturally, the hardware is also compliant with the specifications required by each Pharmacopeia. In addition, the check conditions can be saved. As a result, once the conditions are saved, checks can be performed easily just by calling them up as needed. Check results can also be saved.

Resolution of 1 nm, the Highest in its Class

In addition to achieving a resolution of 1 nm, the highest in its class, by using a monochromator with a Czerny-Turner mounting, the UV-1900i also features a compact, bright optical system. The instrument is more than capable of meeting the wavelength resolution required in the European Pharmacopoeia.

Improved Security Functions

An external control security function has been added to provide more support for compliance with regulations.
Three user authority levels, "Administrator", "Developer", and "Operator", can be set for instrument users.

Low Stray Light

Stray light is at 0.5 % max. (198 nm), twice as low as the performance level of the
UV-1800. With this stray light reduction, accurate measurements are possible up to the vicinity of 2 Abs even in the ultraviolet region. In addition, high-concentration samples can be quantified accurately.
The figure on the right is a calibration curve for acetic acid, created with absorbance at 200 nm.
The correlation coefficient is 0.9997, and correct measured values are obtained even in the vicinity of 2 Abs. Linearity will be lost in the high absorbance region due to the stray light.

Ultra-Fast Scan

Spectra can be acquired as fast as 29,000 nm/min. Ultra-fast scan is effective in tracking chemical reactions in a short time. In addition to the absorbance change at specified wavelengths, spectra can also be acquired in a short time with the UV-1900i. Therefore, more detailed behavior can be investigated by observing spectra with the UV-1900i.
The figures below show the analysis of particle agglomeration process when salts are added to silver nanoparticles. Measurements of the 300 to 700 nm region were performed in ultra-fast scan mode. In addition to the decrease of absorbance at 400 nm and the increase of absorbance at 480 nm, the temporal changes of spectra can also be observed.


Technical Specification



Wavelength range

190 to 1,100 nm

Spectral bandwidth

1 nm (190 to 1,100 nm)

Wavelength display

0.1 nm increments

Wavelength setting

0.1 nm increments

(1 nm increments when setting scanning range)

Wavelength accuracy

± 0.05 nm at D2 peak 656.1 nm,

± 0.3 nm for entire range

Wavelength repeatability

± 0.1 nm

Wavelength slew rate

About 14,500 nm/min

Wavelength scanning speed

3,000 to 2 nm/min

29,000 nm/min when survey scanning

Lamp interchange wavelength

Automatic interchange linked to wavelength.

The interchange wavelength can be set freely in

the range of 295 to 364 nm (0.1 nm increments).

Stray light

Less than 0.02% at 220 nm (NaI)

Less than 0.01% at 340 nm (NaNO²)

Less than 0.5% at 198 nm (KCl)

Photometric system

Double beam optics

Photometric range

Absorbance: -4 to 4 Abs

Transmittance: 0% to 400%

Photometric accuracy

± 0.002 Abs at 0.5 Abs

± 0.0025 Abs at 1.0 Abs

± 0.006 Abs at 2.0 Abs

(measured using NIST930D/NIST1930 or equivalent.)

Photometric repeatability

Less than ± 0.0001 Abs at 0.5 Abs

Less than ± 0.0001 Abs at 1 Abs

Less than ± 0.0005 Abs at 2 Abs




Baseline stability

Less than 0.0003 Abs/Hr

(700 nm, one hour after light source turned ON)

Baseline flatness

Less than ± 0.0006 Abs

(1,100 to 190 nm, one hour after light source turned ON)

Noise level

Less than 0.00003 Abs (700 nm)

Light source

20-W halogen lamp and deuterium lamp

Built-in light source auto position adjustment


LO-RAY-LIGH grade blazed holographic grating in Czerny-Turner mounting


Silicon photodiode

Sample compartment

Internal dimensions: 110 (W) × 250 (D) × 115 (H) mm

Distance between light beams: 100 mm

Power requirements

AC100,120,220,230,240 V,

50/60 Hz, 140 VA



Temperature: 15°C to 35°C

Humidity: 30% to 80%

(without condensation; 70% max. at 30°C or higher)


450 (W) × 501 (D) × 244 (H) mm


16.6 kg

Output device

USB memory (optional)

Data files saved in text format or UVPC format.

UVPC-format files can be read directly by UVProbe and LabSolutionsTM UV-Vis.

PC compatibility

UVProbe software (standard)

LabSolutions UV-Vis software (optional)

External control possible via USB.


24-Bit colour touch screen

Supported Languages

Japanese, English, Chinese, Spanish (Mexico), Portuguese (Brazil).

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