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SPECTROstar OMEGA

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Ultra-fast UV/Vis spectrum absorbance spectrometer - upgradeable to a multi-mode microtiter plate reader

The SPECTROstar^® Omega is a dedicated absorbance microplate reader that can capture a full absorbance spectrum (220 - 1000 nm) in less than 1 second per well – no scanning needed. This upgradeable reader offers the highest flexibility for optimizing settings for all absorbance-based assays. The LVis Plate compatibility allows for low volume sample measurements.

The ultra-fast, dedicated absorbance microplate reader.

Details

The SPECTROstar Omega is a dedicated absorbance microplate reader that can capture either a full-absorbance spectrum or up to eight discrete wavelengths simultaneously. Upgrades can transform SPECTROstar Omega into a powerful multi-mode reader with up to six detection modes, Simultaneous Dual Emission, injectors, and an Atmospheric Control Unit (ACU)

Features

Full-spectrum UV/Vis absorbance measurements in less than 1 second
Measurement in up to 1536-well microplate format
Plate shaking and uniform incubation up to 45 °C or 65 °C
Well-scanning and real-time kinetic monitoring
High-precision injectors with simultaneous reagent injection and detection
Includes multi-user control and MARS data analysis software
Compatible with LVis Plate for low volume samples (down to 2µl)
ACU and stacker compatible

Technical Specs

Technical Specification

Detection Modes	UV/Vis Absorbance Spectra
Measurement Modes	Endpoint and kinetic measurements Spectral scanning (absorbance) Well scanning
Microplate Formats	6 to 1536-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier	Robot compatible
Light Sources	High energy xenon flash lamp
Detectors	CCD Spectrometer
Wavelength Selection	UV/Vis Absorbance Spectrometer Full spectra or 8 distinct wavelengths in < 1 sec / well
Spectral Range	Spectrometer 220 - 1000 nm for ABS
Sensitivity*	ABS with Spectrometer Full spectrum captured in < 1 s/well Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD Reproducibility: ±0.005 OD for 0 - 2 OD range
Read Times	Full spectrum from 220 to 1000 nm in less than 1 sec/well
Reagent Injection	Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 500 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing
Shaking	Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent	System to inject an atmosphere or to pull a vacuum into the reader
Incubation	+4 °C above ambient up to 45 °C or 65 °C The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software	Multi-user Reader Control and MARS Data Analysis Software included FDA 21 CFR Part 11 compliant
Dimensions	Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Optional Accessories
LVis Plate	Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples. Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy Dimensions: Conforms to SBS standards for microplates.
Atmospheric Control Unit (ACU)	Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg
Stacker	Magazines for up to 50 plates - continuous loading feature
THERMOstar	Microplate Incubator and Shaker
*	LOD = 3 x SD (20 blanks) / slope (6 pt std curve) Microplatets: clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates LOD = 3 x SD (20 blanks) / slope (6 pt std curve) Microplates: clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates

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