Multi-mode microplate reader with Simultaneous Dual Emission - including fluorescence polarization

The POLARstar® Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. Equipped with BMG LABTECH’s ultra-fast UV/Vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications

The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with six detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen®/AlphaLISA®. For cell-based applications, the optional Atmospheric Control Unit (ACU) makes O2 and CO2 gas control possible.


  • Two matched PMTs for Simultaneous Dual Emission detection
  • Full-spectrum UV/Vis absorbance measurements in less than 1 second
  • Measurement in up to 384-well microplate format
  • Top and bottom reading
  • Plate shaking and uniform incubation up to 45 °C or 65 °C
  • Well-scanning and real-time kinetic monitoring
  • High-precision injectors with simultaneous reagent injection and detection
  • Includes multi-user control and MARS data analysis software
  • ACU compatible for O2 and CO2 control
  • Compatible with LVis Plate and Stacker
Technical Specification

Detection Modes

  • Fluorescence Intensity
  • FRET
  • Fluorescence Polarization
  • AlphaScreen®/AlphaLISA®
  • Luminescence (flash and glow)
  • BRET
  • Time-Resolved Fluorescence - including TR-FRET
  • UV/Vis Absorbance Spectra

Measurement Modes

  • Top and bottom reading
  • Endpoint and kinetic measurements
  • Sequential Multi Excitation measurements
  • Sequential Multi Emission measurements
  • Simultaneous Dual Emission measurements
  • Spectral scanning (absorbance)
  • Ratiometric measurements
  • Well scanning

Microplate Formats

6 to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp


Two low noise photomultiplier tubes
CCD spectrometer

Wavelength Selection

Optical Filters: Ex and Em wheels for 8 filters each
UV/Vis Absorbance Spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well

Optical Filters

Excitation and emission filter wheels for 8 filters each

Optical Path Guides

Top: Liquid filled light guides
Bottom: Fiber optics

Spectral Range

240 - 750 nm or 240 - 900 nm for FI, FP, TRF
240 - 750 nm for LUM
220 - 1000 nm for ABS


< 10 pM (< 0.2 fmol/well Fluorescein, 384sv, 20 μL)
< 5 mP SD at 1 nM Sodium Fluorescein
< 30 amol/well Europium 
High-End TRF for Omega
< 3 amol/well Europium 
< 20 amol/well ATP 
AlphaScreen® with flash lamp
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS with Spectrometer
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times

Flying mode (1 flash)
9 s (96), 16 s (384)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing


Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader


+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. 


Multi-user Reader Control and MARS Data Analysis Software included.
FDA 21 CFR Part 11 compliant


Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Optional Accessories

LVis Plate

Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy
Dimensions: Conforms to SBS standards for microplates.

Atmospheric Control Unit (ACU)

Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg


Magazines for up to 50 plates - continuous loading feature


Microplate Incubator and Shaker

Optical Filters

Excitation and emission filter wheels for 8 filters each


LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates 
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