Detection Modes
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- Fluorescence Intensity
- FRET
- Fluorescence Polarization
- AlphaScreen®/AlphaLISA®
- Luminescence (flash and glow)
- BRET
- Time-Resolved Fluorescence - including TR-FRET
- UV/Vis Absorbance Spectra
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Measurement Modes
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- Top and bottom reading
- Endpoint and kinetic measurements
- Sequential Multi Excitation measurements
- Sequential Multi Emission measurements
- Simultaneous Dual Emission measurements
- Spectral scanning (absorbance)
- Ratiometric measurements
- Well scanning
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Microplate Formats
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6 to 1536-well plates, user-definable LVis Plate with 16 low volume microspots (2 μL) |
Microplate Carrier
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Robot compatible |
Light Sources
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High energy xenon flash lamp |
Detectors
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Two low noise photomultiplier tubes CCD spectrometer |
Wavelength Selection
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Optical Filters: Ex and Em wheels for 8 filters each UV/Vis Absorbance Spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well |
Optical Filters
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Excitation and emission filter wheels for 8 filters each |
Optical Path Guides
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Top: Liquid filled light guides Bottom: Fiber optics |
Spectral Range
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Filters 240 - 750 nm or 240 - 900 nm for FI, FP, TRF 240 - 750 nm for LUM Spectrometer 220 - 1000 nm for ABS |
Sensitivity*
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FI < 10 pM (< 0.2 fmol/well Fluorescein, 384sv, 20 μL) FP < 5 mP SD at 1 nM Sodium Fluorescein TRF < 30 amol/well Europium High-End TRF for Omega < 3 amol/well Europium LUM < 20 amol/well ATP AlphaScreen® with flash lamp < 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL) ABS with Spectrometer Full spectrum captured in < 1 s/well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD |
Read Times
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Flying mode (1 flash) 9 s (96), 16 s (384) |
Reagent Injection
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Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 500 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing |
Shaking
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Linear, orbital, and double-orbital with user-definable time and speed |
Purge Gas Vent
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System to inject an atmosphere or to pull a vacuum into the reader |
Incubation
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+4 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. |
Software
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Multi-user Reader Control and MARS Data Analysis Software included. FDA 21 CFR Part 11 compliant |
Dimensions
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Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg |
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Optional Accessories
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LVis Plate
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Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples. Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy Dimensions: Conforms to SBS standards for microplates. |
Atmospheric Control Unit (ACU)
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Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg |
Stacker
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Magazines for up to 50 plates - continuous loading feature |
THERMOstar
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Microplate Incubator and Shaker |
Optical Filters
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Excitation and emission filter wheels for 8 filters each |
*
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LOD = 3 x SD (20 blanks) / slope (6 pt std curve) AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates |