NanoDrop Ultra microvolume spectrophotometer

The Thermo Fisher Scientific NanoDrop™ Ultra is the latest generation of microvolume spectrophotometers. The unique Acclaro™ sample intelligence technology uses a sophisticated chemometric approach to identify and correct for copurified contaminants. This helps to reduce the likelihood of downstream reaction failure, saving time, money and troubleshooting.

Operation

• Familiar ‘pipette, measure and wipe workflow’
• 5 Auto-ranging measurement pathlengths
• Measure up to 550 ABS without dilution
• Auto blank and auto measure upon lowering sample arm
• On-board guided troubleshooting
• Camera detection of bubbles with alerts
• Ergonomic tilting 10.1 inch colour touchscreen interface
• Flexible data export via front USBC port, rear USBA, ethernet or Wi-Fi
• USB-C power input allows external battery operation
• Direct Cloud export into Google Drive or Microsoft OneDrive
• Optional 21 CFR part 11 compliant SciVault 2 software can be installed on local control or PC
• RESTful API for seamless LIMS integration

Nucleic Acids

• Measure between 1 - 27,500 ng/µl dsDNA without dilution
• Spectral scans, concentration and purity ratios
• Identification of copurified contaminants with concentration correction
• DNA / RNA differentiation for mammalian, plant or bacterial origins
• qPCR recipe calculator simplifies downstream reaction setup
• Oligo calculator and nucleic acid labelling efficiency determinations

Proteins

• Measure up to 820 mg/ml BSA or 400 mg/ml IgG without dilution
• Protein A280 module for purified proteins
• Detection of copurified nucleic acids with concentration correction
• A205 module for quantification of peptides, or proteins lacking Trp or Tyr residues
• Colorimetric assays for protein mixtures or cell lysates

Other Applications

• Wide wavelength range (190 – 850 nm) for assay flexibility
• OD600 for bacterial cultures
• Custom method capability with on-instrument method editor
• Potential applications include Gold Nanoparticle characterisation, glucose, haemoglobin and chlorophyll

NanoDrop Ultra Cuvette Function

The NanoDrop Ultra C model instrument include an integrated cuvette reader, providing additional assay flexibility, including kinetic assays.

• Measure cuvettes with the sample arm up for convenience
• Integrated heater (37 ˚C) and magnetic stirrer

Acclaro Pro

Unique software algorithms and calibration provide increased accuracy at higher sample concentrations for PAT environments.

TECHNICAL DETAILS:

Instrument Control – Built-in 10.1 inch touchscreen or PC control

Minimum Sample Volume – 1 µL

Limit of Detection – dsDNA Pedestal: 1.0 ng/µL

BSA (IgG) Pedestal: 0.03 (0.032) mg/mL

Maximum Concentration – dsDNA Pedestal: 27,500 ng/µL

BSA (IgG) Pedestal: 820 (400) mg/mL

Photometric Range – 0.02 – 550 Abs (10 mm equivalent)

Photometric Accuracy – 3% at 0.97A, 302 nm

Pathlengths – 1.0, 0.2, 0.1, 0.05 and 0.03 mm auto-ranging

Measurement and Data Processing Time – 7 seconds

Wavelength Range – 190-850 nm

Dimensions (W × D × H) – 32 × 18 × 28 cm (12.6 × 7.1 × 11.0 in.)

Weight – 4.1 kg (9.0 lbs.)

Internal Storage – 64 GB (~500,000 samples)

On-board Control – Custom Linux with 1.6 GHz Quad Core CPU

Power Consumption – 7 W at idle, 11-18 W at Working Conditions

Battery – Supports a USBC external battery pack (75 Wh yields ~8 hours of runtime)

Connectivity – 2 x USB-A ports, 1 x USB-C, 1 x USB-C power input, Ethernet, Bluetooth® and WiFi

Warranty – 2 Years