NanoDrop One / OneC

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The Thermo Fisher Scientific NanoDrop One / OneC are the successors to the ever popular ND1000 and ND2000 models. They are compact standalone microvolume spectrophotometers, with unique Acclaro™ sample intelligence technology, which uses a sophisticated chemometric approach to identify and correct for copurified contaminants. This helps to reduce the likelihood of downstream reaction failure, saving time, money and troubleshooting.



·       Familiar ‘pipette, measure and wipe workflow’

·       5 Auto-ranging measurement pathlengths

·       Measure up to 550 ABS without dilution

·       Auto blank and auto measure upon lowering sample arm

·       On-board guided troubleshooting

·       Camera detection of bubbles with alerts

·       Ergonomic variable angle 7 inch colour touchscreen interface

·       Flexible data export via USB drive, ethernet or Wi-Fi

·       3 USB ports, including one at the front for convenience

·       Free of charge PC software for control or offline viewing of data

·       Optional 21 CFR part 11 compliant Security Suite PC software


Nucleic Acids

·       Measure up to 27,500 ng/µl dsDNA without dilution

·       Spectral scans, concentration and purity ratios

·       Identification of copurified contaminants with concentration correction

·       Mammalian DNA / RNA differentiation

·       Oligo calculator and nucleic acid labelling efficiency determinations


·       Measure up to 820 mg/ml BSA or 400 mg/ml IgG without dilution

·       Protein A280 module for purified proteins

·       Detection of copurified nucleic acids with concentration correction

·       A205 module for quantification of peptides, or proteins lacking Trp or Tyr residues

·       Colorimetric assays for protein mixtures or cell lysates

Other Applications

·       Wide wavelength range (190 – 850 nm) for assay flexibility

·       OD600 for bacterial cultures

·       Custom method capability

·       Potential applications include Gold Nanoparticle characterisation, glucose, haemoglobin and chlorophyll


NanoDrop OneC Cuvette Function

The NanoDrop OneC instrument also includes an integrated cuvette reader, providing additional assay flexibility, including kinetic assays.

·       Measure cuvettes with the sample arm up for convenience

Integrated heater (37 ˚C) and magnetic stirrer

Technical Specification

Instrument Control – Built-in touchscreen or PC control

Minimum Sample Volume – 1 µL

Limit of Detection – dsDNA Pedestal: 2.0 ng/µL
BSA (IgG) Pedestal: 0.06 (0.03) mg/mL


Maximum Concentration – dsDNA Pedestal: 27,500 ng/µL
BSA (IgG) Pedestal: 820 (400) mg/mL


Measurement and Data Processing Time – 8 seconds

Dimensions (W × D × H) – 20 × 25.4 × 32.3 cm (8 × 10 × 12.7 in.)

Weight – 3.6 kg (7.9 lbs.)

Connectivity – Three USB-A ports, Ethernet, Bluetooth® and WiFi

PC Control Software Requirements – Windows® 10 or higher, 64 bit (Professional versions only)

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