LUMIstar OMEGA

Dedicated Luminescence Microplate Reader

The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications, and can be further upgraded to a multi-mode reader.

The high performance, dedicated luminescence microplate reader.

Details

The LUMIstar Omega is a dedicated reader for luminescence measurements, including BRET. The LUMIstar Omega provides highly sensitive readings of all luminescence applications in end-point and kinetic assays. Moreover, a built-in filter wheel and the optional Simultaneous Dual Emission detection make multiple wavelength detection and BRET applications possible.

Features

  • High performance microplate reader for luminescence
  • Measurement at up to eight distinctive wavelengths
  • Top and bottom reading
  • BRET, optional with Simultaneous Dual Emission detection
  • Up to two reagent injectors
  • Upgradeable to FLUOstar Omega or POLARstar Omega including multiple detection modes and UV/Vis spectrometer
  • Powerful MARS Data Analysis Software
Technical Specification

Detection Modes

Luminescence (flash and glow)
BRET

Measurement Modes

Top and bottom reading
Endpoint and kinetic measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Ratiometric measurements

Microplate Formats

6 to 384-well plates, user-definable

Microplate Carrier

Robot compatible

Detectors

Low noise photomultiplier tube

Wavelength Selection

Optical Filters: Em wheels for 8 filters

Optical Filters

Emission filter wheel for 8 filters

Optical Path Guides

Top: Liquid filled light guides
Bottom: Fiber optics

Spectral Range

Filters
240 - 740 nm for LUM

Sensitivity*

LUM
< 20 amol/well ATP

Read Times

Flying mode (1 flash)
20 s (96), 55 s (384)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. 

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg
 

Optional Accessories

Atmospheric Control Unit (ACU)

Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg

Stacker

Magazines for up to 50 plates - continuous loading feature

THERMOstar

Microplate Incubator and Shaker

Optical Filters

Emission filter wheel for 8 filters

*

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
Microplates: white for LUM
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
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