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FLUOstar OMEGA

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Filter-based multi-mode microplate reader

SelectScience Best Drug Discovery Product 2010, the FLUOstar^® Omega is a multimode microplate reader with five detection modes. It utilizes an ultra-fast UV/Vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar Omega is the ideal plate reader for life science applications.

The upgradeable high performance life science microplate reader.

Details

The FLUOstar Omega is a versatile multi-mode microplate reader for five detection modes: fluorescence intensity (including FRET), luminescence (including BRET), UV/Vis absorbance, time-resolved fluorescence (including TR-FRET), and AlphaScreen^®/AlphaLISA^®. For cell-based applications, the optional Atmospheric Control Unit (ACU) makes O₂ and CO₂gas controll possible.

Features

Full-spectrum UV/Vis absorbance measurements in less than 1 second or filter-based absorbance
Measurement in up to 384-well microplate format
Top and bottom reading
Plate shaking and uniform incubation up to 45 °C or 65 °C
Well-scanning and real-time kinetic monitoring
High-precision injectors with simultaneous reagent injection and detection
Includes multi-user control and MARS data analysis software
ACU compatible for O₂ and CO₂ control
Compatible with LVis Plate and Stacker

Technical Specs

Technical Specification

Detection Modes

Fluorescence Intensity

FRET

AlphaScreen^®/AlphaLISA^®

Luminescence (flash and glow)

BRET

Time-Resolved Fluorescence - including TR-FRET

UV/Vis Absorbance Spectra

Measurement Modes

Top and bottom reading

Endpoint and kinetic measurements

Sequential Multi Excitation measurements

Sequential Multi Emission measurements

Spectral scanning (absorbance)

Ratiometric measurements

Well scanning

Microplate Formats

6 to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

Robot compatible

Light Sources

High energy xenon flash lamp

Detectors

Low noise photomultiplier tube
CCD spectrometer

Wavelength Selection

Optical Filters: Ex and Em wheels for 8 filters each
UV/Vis Absorbance Spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well

Optical Filters

Excitation and emission filter wheels for 8 filters each

Optical Path Guides

Top: Liquid filled light guides
Bottom: Fiber optics

Spectral Range

Filters	240 - 750 nm or 240 - 900 nm for FI, FP, TRF 240 - 750 nm for LUM
Spectrometer	220 - 1000 nm for ABS

Sensitivity*

FI	< 0.2 fmol/well Sodium Fluorescein
TRF	< 30 amol/well Europium
High-End TRF for Omega	< 3 amol/well Europium
LUM	< 20 amol/well ATP
AlphaScreen^® with flash lamp	< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS with Spectrometer	Full spectrum captured in < 1 s/well Selectable spectral resolution: 1, 2, 5, and 10 nm OD range: 0 to 4 OD Accuracy: < 1% at 2 OD Precision: < 0.5% at 1 OD and < 0.8% at 2 OD

Read Times

Flying mode (1 flash)
9 s (96), 16 s (384)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

Optional Accessories

LVis Plate

Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.

Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy

Dimensions: Conforms to SBS standards for microplates.

Atmospheric Control Unit (ACU)

Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg

Stacker

Magazines for up to 50 plates - continuous loading feature

THERMOstar

Microplate Incubator and Shaker

Optical Filters

Excitation and emission filter wheels for 8 filters each

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen^® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen^®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates

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BUYING OPTIONS

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Product Code	Description	List Price	Offer Price
415-102-AF	FLUOstar OMEGA, Standard & Time Resolved Fluorescence, Absorbance, Measurement in up to 384-well microplate format, Top and bottom reading, Plate shaking and uniform incubation up to 45	POA	POA	ENQUIRE
415-102-AFL	FLUOstar OMEGA, Standard & Time Resolved Fluorescence, Absorbance and High Performance Luminescence, Measurement in up to 384-well microplate format, Top and bottom reading, Plate shaking and uniform incubation up to 45	POA	POA	ENQUIRE
415-101-AF	FLUOstar OMEGA, Standard & Time Resolved Fluorescence and Absorbance, Plate shaking and uniform incubation up to 45	POA	POA	ENQUIRE
415-101-AFL	FLUOstar OMEGA, Standard & Time Resolved Fluorescence, Absorbance and Luminescence*, Plate shaking and uniform incubation up to 45	POA	POA	ENQUIRE

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